Understanding the Regulation of Early Mouse Embryonic Development

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In this project theme, our lab is interested in understanding how normal mouse embryonic development is regulated by both transcription factors and long non coding RNAs. Using embryonic stem cells and embryoid body differentiation, a common model of early mouse development, we are able to perform in depth studies on critical early developmental processes. These processes include: the maintainance of pluripotency, early cell fate decisions and in particular the regulation of gastrulation, a key developmental process that establishes the basis for all of the tissues of the adult organism. We are able to study these processes using techniques such as: CRISPR-Cas9 genome editing, shRNA knockdown, RNAseq, ChIPseq, in situ hybridization and imaging.