CRISPR-Cas9 genome editing is a revolutionary technology that allows rapid and precise modification of the eukaryotic genome. Developed in 2013, the technology has exploded, with over 1000 published articles using the technology. Given the simplicity and efficiency of the technology, our lab has adopted it for a variety of projects.
We have developed protocols for CRISPR-Cas9 design, reagent preparation and mutant analysis. Our protocols aim to be high throughput to reduce laborious tasks and cost.